Small Rna Library Prep Kit

The unique workflow of the nebnext small rna library prep kits addresses the challenge of minimization of adaptor dimers while achieving production of high yield diverse multiplex libraries in a simple protocol.

Small rna library prep kit.

The nebnext multiplex small rna library prep kit for illumina index primers 1 48 contains the adaptors primers enzymes and buffers required to convert small rnas into indexed libraries for next generation sequencing on the illumina platform. Truseq small rna library prep kit reference guide 15004197 instructions for preparing libraries using the truseq small rna library prep kit. This version of the kit is intended for use with your barcode system and please note that index primers for multiplexing are not included in this kit. The human brain reference rna hbrr and universal human reference rna uhrr samples were prepared using the truseq small rna library prep kit.

The kit is available in 3 sizes with 8 24 and 96 preps. Multiplexing of up to 96 samples multiplexing of up to 96 samples is possible with complimentary i7 indexes provided in the kit. The small rna seq library prep kit provides reagents for generating small rna libraries for illumina sequencing directly from total rna or enriched small rna. It includes all the necessary reagents for the complete workflow including barcodes for multiplexing.

The total yield was 0 91 gb with 97 of bases at or above q30. The small rna seq library prep kit provides a protocol for generating small rna libraries for illumina sequencing directly from total rna or enriched small rna. The novel workflow has been optimized to minimize adaptor dimers while producing high yield high diversity libraries.